Data containers

  • The core of software
  • Structured, standardized way to manage complex data
  • Enables modular, efficient workflows

Optimal container for microbiome data?

Optimal container for microbiome data?

  • Multiple assays: seamless interlinking

Optimal container for microbiome data?

  • Multiple assays: seamless interlinking
  • Hierarchical data: supporting samples & features

Optimal container for microbiome data?

  • Multiple assays: seamless interlinking
  • Hierarchical data: supporting samples & features
  • Side information: extended capabilities & data types

Optimal container for microbiome data?

  • Multiple assays: seamless interlinking
  • Hierarchical data: supporting samples & features
  • Side information: extended capabilities & data types
  • Optimized: for speed & memory

Optimal container for microbiome data?

  • Multiple assays: seamless interlinking
  • Hierarchical data: supporting samples & features
  • Side information: extended capabilities & data types
  • Optimized: for speed & memory
  • Integrated: with other applications & frameworks

Optimal container for microbiome data?

  • Multiple assays: seamless interlinking
  • Hierarchical data: supporting samples & features
  • Side information: extended capabilities & data types
  • Optimized: for speed & memory
  • Integrated: with other applications & frameworks

Reduce overlapping efforts, improve interoperability, ensure sustainability.

TreeSummarizedExperiment

(Huang et al. 2021)

TreeSummarizedExperiment class

SummarizedExperiment

(Huber et al. 2015)

MultiAssayExperiment

(Ramos et al. 2017)

  • For more complex sample mapping
TreeSummarizedExperiment class

Demonstration

print(tse)
class: TreeSummarizedExperiment 
dim: 19216 26 
metadata(0):
assays(1): counts
rownames(19216): 549322 522457 ... 200359 271582
rowData names(7): Kingdom Phylum ... Genus Species
colnames(26): CL3 CC1 ... Even2 Even3
colData names(7): X.SampleID Primer ... SampleType Description
reducedDimNames(0):
mainExpName: NULL
altExpNames(0):
rowLinks: a LinkDataFrame (19216 rows)
rowTree: 1 phylo tree(s) (19216 leaves)
colLinks: NULL
colTree: NULL

assay(tse, "counts")
         CL3   CC1   SV1 M31Fcsw M11Fcsw M31Plmr M11Plmr F21Plmr M31Tong
549322     0     0     0       0       0       0       0       0       0
522457     0     0     0       0       0       0       0       0       0
951        0     0     0       0       0       0       1       0       0
244423     0     0     0       0       0       0       0       0       0
586076     0     0     0       0       0       0       0       0       0
246140     0     0     0       0       0       0       0       0       0
143239     7     1     0       0       0       0       3       0       0
244960     0     0     0       0       0       0       0       0       0
255340   153   194     0       0       0       0       0       0       0
144887     3     5     0       0       0       0       0       0       0
141782     9    35     0       0       0       0       0       0       0
215972     0     3     0       0       0       0       0       0       0
31759      0     1     0       0       0       0       0       0       0
30678      0     3     0       0       0       0       0       0       0
138353     0     0    19       0       0       0       0       0       0
406058     1     0     0       0       0       0       0       0       0
30405    808    11     3       0       0       0       0       0       0
1126       0     0     0       0       0       0       0       0       0
46043      0     0     0       0       0       0       0       0       4
512519     0     0     0       0       0       0       0       0       0
554594     0     0     0       0       0       0       0       0       0
210741     0     0     0       0       0       0       0       0       0
550229     0     0     0       0       0       0       0       0       1
105798     0     0     0       0       0       0       0       0       0
183473     0     0     0       0       0       0       0       0       0
541313     0     0     0       0       0       0       0       0       0
540305     0     0     0       0       0       0       0       0       1
511243     0     0     0       0       0       0       0       0       0
568829     0     0     0       0       0       0       0       0       0
185612     0     0     0       0       0       0       0       0       0
321018     0     0     0       0       0       0       0       0       0
549041     0     0     0       0       0       0       0       0       0
153762     0     0     0       0       0       0       0       0       0
155789     0     0     0       0       0       0       0       0       0
155495     0     0     0       0       0       0       0       0       0
1029       0     0     0       0       0       0       0       0       0
155526     0     0     0       0       0       0       0       0       0
197473     0     0     0       0       0       0       0       0       0
315545     0     0     0       0       0       0       0       1      10
348549     0     0     0       1       0       0       1       0       8
559394     0     0     0       0       0       0       0       0       0
341551     0     0     0       0       0       0       0       0       0
108964     0     1     0       0       0       0       2       6       1
330416     0     0     0       0       0       0       0       0       0
573586     0     0     0       0       0       0       0       0       0
568724     0     0     0       0       0       0       0       0       0
175045     0     0     0       0       1       0       0       2       0
552540     0     0     0       0       0       0       0       0       0
546313    72   153 11232       0       1       1       0       1       1
548602     0     0    16       0       0       0       0       0       0
564501     0     0     3       0       0       0       0       0       0
47778      1    14   207       0       0       0       5       0       0
54107      2    87   746       0       0       0       3      35       0
25116      1     4   169       0       0       0       0       1       0
71074     93   341 11788       1       0      23      48      58       2
252853     0     0     0       0       0       0       0       0       0
100679     6    72  1709       0       0       5      11      19       0
540349    16    25  1271       0       0       0       1       0       0
1140       0    18   124       0       0       0       0       1       0
101071    18     0     3       0       0      11       0       8       0
1143       1    11     1       0       0       0       0       0       0
141739     1    39     2       0       0       0       0       0       0
1141       0     4    11       0       0       0       0       0       0
237125     0     7    75       0       0       0       3       1       0
1144       8    71   474       0       0       0       0       1       0
512943     7    28   296       0       0       0       0       0       0
235345     0    22     0       0       0       0       0       0       0
141417     8    87   647       0       0       0       4       3       1
268471     0     0     0       0       0       0       0       0       0
395774     0     0     0       0       0       0       0       0       0
142115     0     0     0       0       0       0       0       0       0
239672     0     0     0       0       0       0       0       0       0
462844     0     0     0       0       0       0       0       1       0
243675     0     0     0       0       0       0       0       0       0
444679     0     0     0       0       0       0       0       0       0
540290     0     0     0       0       0       0       0       0       0
523571     0     0     0       0       0       0       0       0       0
545095     0     0     0       0       0       0       0       0       0
105679     0     0     0       0       0       0       0       0       0
105066     0     0     0       0       0       0       0       0       0
515962     0     0     0       0       0       0       0       0       0
525569     0     0     0       0       0       0       0       0       0
238793     0     0     0       0       0       0       0       0       0
538819     0     0     0       0       0       0       0       0       0
515691     0     0     0       0       0       0       0       0       0
525464     0     0     0       0       0       0       0       0       0
529521     0     0     0       0       0       0       0       0       0
202343     0     0     0       0       0       0       0       0       0
455926     0     0     0       0       0       0       0       0       1
246371     0     0     0       0       0       0       0       0       0
282132     0     0     0       0       0       0       0       0       0
254458     0     0     0       0       0       0       0       0       0
245697     0     3     0       1       1       0       0       0       2
45742      0     0     0       0       0       0       0       0       0
441638     0     0     0       0       0       0       0       0       0
251993     0     0     0       0       0       0       0       0       0
462098     0     0     0       0       0       0       0       0       0
570593     0     0     0       0       0       0       0       0       0
159421     0     0     0       0       0       0       0       0       0
104332     0     0     0       0       0       0       0       0       0
137608     0     0     0       0       0       0       0       0       0
200619     0     0     0       0       0       0       0       0       0
568129     0     0     0       0       0       0       0       0       0
348720     0     0     0       0       0       0       0       0       0
528115     0     0     0       0       0       0       0       0       0
257655     0     0     0       0       0       0       0       0       0
173903     0     0     0       0       0       0       0       0       0
47016      0     0     0       0       0       0       0       0       0
548723     0     0     0       0       0       0       0       0       0
542636     0     0     0       0       0       0       0       0       0
526502     0     0     0       0       0       0       0       0       0
311512     0     0     0       0       0       0       0       0       0
132088     0     0     0       0       0       0      16       0       0
202347     0     0     0       0       0       0       0       0       0
152431     0     0     0       0       0       0       0       0       0
272691     0     0     0       0       0       0       0       0       0
236606     0     0     0       0       0       0       0       0       0
525        0     0     0       0       0       0       0       0       0
52710      0     0     0       0       0       0       0       0       0
592689     0     0     0       0       0       0       0       0       0
55681      0     0     0       0       0       0       0       0       0
206978     0     0     0       0       0       0       0       0       0
467541     0     0     0       0       0       0       0       0       0
566371     0     0     0       0       0       0       0       0       0
453468     0     0     0       0       0       0       0       0       0
557121     0     0     0       0       0       0       0       0       0
524986     0     0     0       0       0       0       0       0       0
102663     0     0     0       0       0       0       0       0       0
264985     0     0     0       0       0       0       0       0       0
37119      0     0     0       0       0       0       0       0       0
349670     0     0     0       0       0       0       0       0       0
240012     0     0     0       0       0       0       0       0       0
248224     0     0     0       0       0       0       0       0       0
236057     0     0     0       0       0       0       0       0       0
236619     0     0     0       0       0       0       0       0       0
565033     1     0     0       0       0       0       0       0       0
212360    10     2     4       0       0       0       0       0       0
146124     0     0     0       0       0       0       0       0       0
251552     0     0     0       0       0       0       0       0       0
542257     0     0     0       0       0       0       0       0       0
548984     0     0     0       0       0       0       0       0       0
201596     0     0     0       0       0       0       0       0       0
158026     0     0     0       0       0       0       0       0       0
62881      0     0     0       0       0       0       0       0       1
329648     0     0     0       0       0       0       0       0       0
570725     0     0     0       0       0       0       0       0       0
206080     0     0     0       0       0       0       0       0       0
547371     0     0     0       0       0       0       0       0       0
140729     0     0     0       0       0       0       0       0       0
347836    15     0     6       0       0       0       0       0       0
76707      0     0     0       0       0       0       0       0       0
136107     2     0     1       0       0       0       0       0       0
170941     4     3     0       0       0       0       0       0       0
155703     0     0     1       0       0       0       0       0       0
213630     6     0     0       0       0       0       0       0       0
546805     0     0     0       0       0       0       0       0       0
448625     0     0     0       0       0       0       0       0       0
101828     0     0     0       0       0       0       0       0       0
319002     3     0     0       1       0       0       0       0       3
219191     0     0     0       0       0       0       0       0       0
410763     0     0     0       0       0       0       0       0       0
412056     0     0     0       0       0       0       0       0       0
550661     0     0     0       0       0       0       0       0       0
550655     0     0     0       0       0       0       0       0       0
526        0     0     0       0       0       0       0       0       0
515698     0     0     0       0       0       0       0       0       0
379145     0     0     0       0       0       0       0       0       0
245798     0     0     0       0       0       0       0       0       0
583469     0     0     0       0       0       0       0       0       0
254858     0     0     0       0       0       0       0       0       0
407348     0     0     0       0       0       0       0       0       0
250392     2     2     3       2       0       2      11       1     194
148407     0     0     0       0       0       0       0       0       0
104364     0     0     0       0       0       0       0       0       0
250570     0     0     0       0       0       0       0       0       0
308755     0     0     0       0       0       0       0       0       4
147601     0     0     0       0       0       0       0       0       0
255494     1     0     0       0       0       0       2       0      30
146866     1     0     0       0       0       0       0       1      13
155847     0     0     0       0       0       0       0       0      15
715        0     0     0       0       0       0       0       0       5
32110      0     0     0       0       0       0       0       0       0
140297     0     0     0       0       0       0       0       0       0
105600     0     0     0       0       0       0       0       0       0
40123      0     0     0       0       0       0       0       0       0
79         0     0     0       0       0       0       0       0       0
18599      0     0     0       0       0       0       0       0       0
77878      1     0     0       0       0       0       1       0       2
101553     0     0     0       0       0       0       0       0       0
204        0     0     0       0       0       0       0       0       0
134        0     0     0       0       0       0       0       0       0
551498     0     0     0       0       0       0       0       0       0
99797      0     0     0       0       0       0       0       0       0
150294     0     0     0       0       0       0       0       0       0
155634     0     0     0       0       0       0       0       0       0
153917     0     0     0       0       0       0       0       0       0
348180     0     0     0       0       0       0       0       0       0
138        0     0     0       0       0       0       0       0       0
547463     0     0     0       0       0       0       0       0       0
152271     1     0     0       0       0       0       0       0       0
227785     0     1     0      27      54       0       0       0       4
104804     0     0     0       0       0       0       0       0       0
128        0     0     0       0       0       0       0       0       0
228569     0     0     0       0       0       0       0       0       0
100449     0     0     0       0       0       0       0       0       0
262662     0     0     0       0       0       0       0       0       0
246686     0     0     0       0       0       0       0       0       0
166443     0     0     0       0       0       0       0       0       0
368907     0     0     0       0       0     254     292      20       1
12724      0     0     0       0       0       2       1       0       0
93749     10     0     0       0       0       1       0       0       0
378053     0     0     0       0       0       3       0       0       0
165106     0     1     0       0       0       0       0       0       0
135424     0     1     0       0       0       0       0       0       0
167566     7    54     4       0       0       2       1       3       0
12751      8   298     4       0       0       0       0       0       0
12753      0    10     0       0       0       0       0       0       0
560734     0     1     3       0       0       0       1       0       0
231733     0     1     0       0       0       0       0       1       0
145101     0     0     0       0       0       0       1       0       0
367851     0     0     1       0       0       0       0       0       0
275357     0     0     1       0       0       0       0       0       0
315847     2    16    30       0       0       0       3       0       0
428663     0     0     1       0       0       0       0       0       0
165629     0     8    48       0       0       0       0       0       0
243161     1     2    23       0       0       0       0       0       0
525323     0    19    27       0       0       0       2       1       0
157893     4    32    17       0       0       4       1       3       0
172332     0    19    19       0       0       2       6       2       0
179479     2    19     0       0       0       0      10       1       1
145776    14   290   187       0       0      28      32      24       0
263308    33    85   226       0       0      12      62      29       0
341901     4    33   217       0       0       1      12       0       0
237339    46   308   227       0       0       1      18       6       0
216616     0    13     6       0       0       0       0       0       0
549341     0     9   115       0       0       0       2       0       0
132361     7     1     5       0       0       0       0       0       0
377237    20    43    32       0       0       0       1       3       0
101707     1     7     7       0       0       2       1       0       1
552729     4    30    25       0       0       6      45      24       1
34725      0    25    11       0       0       5      12       3       0
205152     3     4     4       0       0       0       1       1       0
586825    69   146   432       0       0      31      41      17       0
255802    12     8     1       0       0       0       0       1       0
98983      2    21     3       0       0       0       0       0       0
101669    26   417     3       1       0       6      27       2       0
204284    60   201    39       0       0      10      71      22       0
501795     0     3    77       0       0       0      26       0       0
207298    17    33   513       0       0       2      15       3       0
516593     5    24   127       0       0       0       2       5       0
154684    17    29   185       0       0       1      18       6       0
243943    19    81    65       0       0      10      19      16       0
101852    15    23    12       1       0       3       1       1       0
325277     1    39     1       0       0       2       2       1       0
167263     0     0     5       0       0       0       0       0       0
563475    13   122    36       0       0       2       0       0       0
214797     1     8     0       0       0       0       1       0       0
360170     1     1     0       0       0       0       1       0       0
151383     8    30   218       0       0      18      11      21       0
16538      3    10    29       0       0      14       8       2       0
154113     6    12    82       0       0      12      67      23       2
236362     6    15   124       0       0       2      23       5       0
12762      0     0     0       0       0       0       1       0       0
559914    11    62    96       1       0       4      41       0       0
244158     3     3    10       0       0       2       1       1       0
533951     1     0     2       0       0       2       0       0       0
286030     0     0     0       0       0       0       7       0       0
137567     0    18    77       0       0       8       2       0       0
517399     2     0     1       0       0      35       0      10       0
12778      1     0     0       0       0       0       0       0       0
12776      5    80     2       0       0       0      12       5       0
257075     3    17    39       0       0       2      11       2       1
264145     1     0     1       0       0       0       0       0       0
249247     0     0     1       0       0       0       0       0       0
251908     1     4    35       0       0       1       0       0       0
513390     9   174    14       0       0      17      41       9       0
99516      5     0     0       0       0       1       5       0       0
45520     20     0     0       0       0       1       0       0       0
12789     21     0     1       0       0       1       0       0       0
140932     2     0     0       0       0       1       0       0       0
576124     3    37    23       0       0       1       6       4       0
554391     0     0     2       0       0       0       2       0       0
574022     0     2     1       0       0       3       5       2       0
255749     2     7    38       0       0       6      32       4       0
166467     0     0     1       0       0       0       0       0       0
217144     1    31    46       0       0      14      47       3       0
414150     1    41   179       0       0       0       0       0       0
531515    15    76   109       0       0      12      64      19       0
365828    35   162   239       0       0      26     104      48       0
197085    35    49    62       0       0       2       9       2       0
295675     0     1     0       0       0       0       0       0       0
549557     0    24    20       0       0       1      11       9       0
213587    53   132   219       0       0       8      43       8       0
12787      0     3    14       0       0       3       0       2       0
114316     0    15    15       0       0       0       0       0       0
246385     0     3     0       0       0       0       0       0       0
137878     6    52    82       0       1      31     111      95       2
157872     3    55    24       0       0       1       0       2       0
208818     9   154    42       0       0       6      80      39       0
239739     6     9   132       0       0       3      35      16       0
218299     0     2    25       0       0       1       1       0       0
158804     9    84    28       0       0       8      47       8       0
32247      7   144     3       0       0       9      41       8       0
305897    24    39   726       0       0       2       1       1       0
248838    14    21   957       0       0       0       0       0       0
411799     0     0     8       0       0       0       0       0       0
525694     0     0     0       0       0       0       0       1       0
340457     0     0     4       0       0       0       0       0       0
45777     11     0     0       0       0       0       0       0       0
591315     7    21    60       0       0       0       3       1       0
248170    19   198   528       0       0       1       4       3       1
584943     1     0    44       0       0       0       0       0       0
542225     0     0     0       0       0       0       0       0       0
566820     0     0     0       0       0       0       0       0       0
200104     0     0     0       0       0       0       0       0       0
275402     0     0     0       0       0       0       0       0       0
321069     0     0     0       0       0       0       0       0       0
134809     0     0     0       0       0       0       0       0       0
580386     0     0     0       0       0       0       0       0       0
517376     0     0     0       0       0       0       4       2       0
534601     0     0     1       0       0       0       0       0       0
587114     0     0     0       0       0       0       0       0       0
203278     0     0     0       0       0       0       0       0       0
536016     0     0     0       0       0       0       0       0       0
552440     0     0     0       0       0       0       0       0       0
591611     0     0     0       0       0       0       0       0       0
146727     0     0     0       0       0       0       0       0       0
350544     0     0     0       0       0       0       0       0       0
356778     0     0     0       0       0       0       0       0       0
571148     0     0     0       0       0       0       0       0       0
354609     0     0     0       0       0       0       0       0       0
514700     0     0     0       0       0       0       0       0       0
210359     0     0     0       0       0       0       0       0       0
210533     0     0     0       0       0       0       0       0       0
169682     0     0     0       0       0       0       0       0       0
575324     0     0     0       0       0       0       0       0       0
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543491     1     3     3       2       7    1860      91     172   15226
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563047     0     2     7       1       4    7868      52    1219     303
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370121     2     0     0       0       0       0       0       0       0
149350     0     0     0       0       0       0       0       0       0
469903     0     0     0       0       0     533      13     177      13
65940      0     0     0       1       0     781       3     214      13
211976     0     0     0       0       0       0       0       0       0
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173632     0     0     2       0       0      35      87       2       2
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109054     5     0     0       0       0       5       0       0       4
309588     5     0     0       0       0       4       1       0       0
50424      0     0     0       0       0       0       0       0       6
256595     0     0     0       0       0       0       0       0       0
38060      0     0     0       0       0       0       0       0       0
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198608     0     0     0       0       0       0       0       0       0
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272031     0     0     0       0       0       0       0       0       0
357417     1     0     0       0       1       0       6       0      15
73722      0     0     0       0       0       0       0       0       0
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359108     0     0     0       0       0       0       0       0       3
140761     0     0     0       0       0       9       0       4       2
259542     0     0     0       0       0      11       0       3       0
274227     0     0     0       0       0       0       0       1       0
71138      0     0     0       0       0       0       0       0       0
140774     0     0     0       0       0       4       0       2       1
153557     0     0     0       0       0       0       0       0       0
275521     0     0     0       0       0       0       0       0       0
84701      0     0     0       0       0       0       0       0       0
140981     0     0     0       0       0       0       0       0       0
199155     0     0     0       0       0       1       0       1       0
140915     0     0     0       0       0       0       0       0       0
126847     0     0     0       0       0       0       0       0       0
161179     0     0     0       0       0       3       0       2       1
140854     0     0     0       0       0       1       0       0       0
140783     0     0     0       0       0       0       0       0       0
140388     0     0     0       0       0       0       0       0       0
140818     0     0     0       0       0       0       0       0       0
140920     0     0     0       0       0       0       0       0       0
140829     0     0     0       0       0       0       0       0       0
140967     0     0     0       0       0       0       0       0       0
79171      0     0     0       0       0       0       0       0       0
140805     1     0     0       0       0       0       0       0       0
246147     0     0     0       0       0       0       0       0       0
545268     0     0     0       0       0       0       0       0       0
193662     0     0     0       0       0       0       1       0       1
160023     1     0     0       0       1       0       0       0       1
39185      0     2     0       0       0       0       0       0       0
98089      0     0     0       0       0       0       0       0       0
71948      0     0     0       0       0       0       0       0       0
259571     0     0     0       0       0       0       0       0       0
319701     1     0     0       0       0       0       0       0       0
323793     0     0     0       0       0       0       0       0       0
37948      0     0     2       0       0       0       0       0       0
316234    21     1     0       0       0       0       0       0       0
377895     0     0     0       0       0       0       0       0       0
387053     0     0     0       0       0       0       0       0       0
268666     0     0     0       0       0       0       0       0       0
144284     0     0     0       0       0       0       0       0       0
330658     0     0     0       0       0       0       0       0       0
214514     0     0     0       0       0       0       0       0       0
359602     0     0     0       0       0       0       0       0       0
262781     0     0     0       0       0       0       0       0       0
382239     0     0     0       0       0       0       0       0       0
131602     0     0     0       0       0       0       0       0       0
246806     0     0     0       0       0       0       0       0       0
230971     0     0     0       0       0       0       0       0       0
81968      0     0     0       0       0       0       0       0       0
365740     0     0     0       0       0       0       0       0       0
322290     0     0     0       0       0       0       0       0       0
368438     0     0     0       0       0       0       0       0       0
594414     0     0     0       0       0       0       0       0       0
322845     0     0     0       0       0       0       0       0       0
8864       0     0     0       0       0       0       0       0       0
268501     0     0     0       0       0       0       0       0       2
553622     0     0     0       0       0       0       0       0       0
163227     0     0     0       0       0       0       0       0       0
81643      0     0     0       0       0       0       0       0       0
531807     0     0     0       0       0       0       0       0       0
146764     0     0     0       0       0       0       0       0       0
158539     0     0     0       0       0       0       0       0       0
248182     0     0     0       0       0       0       0       0       0
235556     0     0     0       0       0       0       0       0       0
392867     0     0     0       0       0       0       0       0       1
8849       0     0     0       0       0       0       0       0       0
8854       0     0     0       0       0       0       0       0       0
272511     0     0     0       0       0       0       0       0       1
334678     0     0     0       0       0       0       0       0       0
46271      0     0     0       0       0       0       0       0       1
319651     0     0     0       0       0       0       0       0       0
255639     0     0     0       0       0       0       0       0       0
68895      0     0     0       0       0       0       0       0       0
108410     0     0     0       0       0       0       0       0       0
230360     0     0     0       0       0       0       0       0       0
71462      0     0     0       0       0       0       0       0       0
82812      0     0     0       0       0       0       0       0       0
325564     0     0     0       0       0       0       0       0       0
230735     0     0     0       0       0       0       0       0       0
224717     0     0     0       0       0       0       0       0       0
21967      0     0     0       0       0       0       0       0       0
362563     0     0     0       0       0       2      40       0       1
346453     0     0     0       0       0       0       0       0       0
586740     0     0     0       0       0       1      27       1       0
260031     0     0     0       0       0       0       1       0       0
236190     0     0     0       0       0       0       0       0       0
356544     0     0     0       0       0       0       0       0       0
228086     0     0     0       0       0       0       0       0       0
70306      0     0     0       0       0       0       0       0       0
128050     0     0     0       0       0       0       1       0       0
245610     0     0     0       0       0       0       0       0       0
141151     0     0     0       0       0       0       1       0       0
554703     0     0     0       0       0       0       0       0       0
169940     0     0     0       0       0       0       1       0       0
93965      0     0     0       0       0       0       0       0       0
277789     0     0     0       0       0       0       0       0       0
106027     0     0     0       0       0       0       0       0       0
91588      0     0     0       0       0       0       0       0       0
523223     0     0     0       0       0       0       0       0       0
536526     0     0     0       0       0       0       0       0       0
84937      0     0     0       0       0       2      23       0       0
9286       0     0     0       0       0       0       1       0       1
60374      0     0     0       0       0       0       0       0       0
327981     0     0     0       0       0       0       0       0       0
234088     0     0     0       0       0       0       0       0       0
353304     0     0     0       0       0       0       0       0       0
536960     0     0     0       0       0       0       0       0       0
9176       0     0     0       0       0       0       0       0       0
91087      0     0     0       0       0       0       0       0       0
198609     0     0     0       0       0       0       0       0       0
9179       0     0     0       0       0       0       0       0       0
231428     0     0     0       0       0       1       1       0       0
559028     0     0     0       0       0       0       0       0       0
232634     0     0     0       0       0       0       0       0       0
230146     0     0     0       0       0       0       0       0       0
30925      0     0     0       0       0       0       0       0       1
111212     0     0     0       0       0       0       0       0       0
549366     0     1     0       0       1       0       0       0       2
233630     0     0     0       0       0       0       0       0       0
220372     0     0     0       0       0       0       0       0       0
250349     0     0     0       0       0       0       1       0       0
339560     0     0     0       0       0       0       0       0       0
579117     0     0     0       0       0       0       0       0       0
9250       0     0     0       0       0       0       0       0       0
559495     0     0     0       0       0       0       0       0       0
320634     0     0     0       0       0       0       0       0       0
306938     0     0     0       0       0       1      36       0       0
534155     0     0     0       0       0       0       0       0       0
196433     0     0     0       0       0       0       0       0       0
553472     0     0     0       0       0       0       0       0       0
573179     0     0     0       0       0       0       0       0       0
227760     0     0     0       0       0       2      16       0       0
160210     0     0     0       0       0       0       0       0       0
579828     0     0     0       0       0       0       0       0       1
171395     0     0     0       0       0      14       0       0       0
160207     0     0     0       0       0     686      44       0       0
159955     0     0     0       0       0       0       0       0       0
245715     0     0     0       0       0       0       0       0       0
351250     0     0     0       0       0      25       2       0       0
567653     0     0     0       0       0    1134     103       0       2
114914     0     0     0       0       0       0       0       0       0
160550     0     0     0       0       0       1       0       0       0
20588      0     0     0       0       0       0       0       0       0
132680     0     0     0       0       0       0       0       0       0
570650     0     0     0       0       0       1       0       0       0
9142       0     0     0       0       0       0       2       0       0
160832     0     0     0       0       0       0       0       0       0
549933     0     0     0       0       0      21       0       0       0
111916     0     0     0       0       0       1       0       0       0
45389      0     0     0       0       0       0       0       0       0
9129       0     0     0       0       0       1       0       0       0
9128       0     0     0       0       0      12       0       0       0
149694     0     0     0       0       0       0       0       0       0
236039     0     0     0       0       0       0       1       0       0
246710     0     0     0       0       0       0       0       0       0
9036       0     0     0       0       0       0       0       0       0
349841     0     0     0       0       0       0       0       0       0
267927     0     0     0       0       0       0       0       0       0
229619     0     0     0       0       0       0       0       0       0
83672      0     0     0       0       0       0       0       0       0
346602     0     0     0       0       0       0       0       0       0
143206     0     0     0       0       0       0       0       0       0
148004     0     0     0       0       0       0       0       0       0
151850     0     0     0       0       0       0       0       0       1
179131     0     1     0       0       0       0       0       0       0
132764     0     0     0       0       0       0       0       0       0
133572     1     1     0       0       0       0       0       0       0
38945      2     0     0       0       0       0       0       0       0
113783     0     0     0       0       0       0       0       0       0
276135     0     1     0       0       0       1       8      20       1
141639     0     0     0       0       0       0       0       0       0
173543     0     0     0       0       0       0       0       0       0
238071     0     0     0       0       0       1       0       0       0
586719     0     0     0       0       0       0       0       0       0
139040     0     0     0       0       0       0       0       0       0
140647     0     0     0       0       0       0       0       0       0
256999     0     0     0       0       0       0       0       0       1
155961     0     0     0       0       0       0       0       0       0
168803     0     0     0       0       0       0       0       0       0
341133     0     0     0       0       0       0       0       0       0
128057     0     0     0       0       0       0       0       0       0
9123       0     0     0       0       0       0       0       0       0
237124     1     0     0       0       0       0       0       0       0
571322     0     0     0       0       0       0       0       0       0
252198     0     0     0       0       0       0       0       0       3
543247     0     0     0       0       0       0       0       0       0
104600     0     0     0       0       0       0       0       0       0
576068     0     0     0       0       0       0       0       0       0
357877     1     0     0       0       0       0       2       0       1
168069     0     3     0       1       1       1     162      11       0
161901     0     0     0       0       0       0       0       0       0
215466     0     0     0       0       0       0       0       0       0
423025     0     0     0       0       0       0       0       0       0
509473     0     0     0       0       0       0       0       0       0
9458       0     0     0       0       0       0       0       0       0
583215     0     0     0       0       0       0       0       0       1
578335     0     0     0       0       0       0       0       0       0
9459       0     0     0       0       0       0       0       0       0
70512      1     0     0       0       0      37       3       2     379
9514      11    28     8      60      13   11162     291    2422  140781
29369      0     0     0       0       0       0       0       0       0
471414     0     0     0       0       0       0       0       0       0
9466       0     0     0       0       0       0       2       0       2
286210     0     0     0       0       0       5      27       5       0
       M11Tong LMEpi24M SLEpi20M AQC1cm  AQC4cm AQC7cm   NP2    NP3    NP5
549322       0        0        1     27     100    130     1      0      0
522457       0        0        0      0       2      6     0      0      0
951          0        0        0      0       0      0     0      0      0
244423       0        0        0      0      22     29     0      0      0
586076       0        0        0      0       2      1     0      0      0
246140       0        0        0      0       1      3     0      0      0
143239       0        0        0      0       0      0     0      0      0
244960       0        0        0      0       1      0     0      0      0
255340       0        0        0      1       0      1     0      0      0
144887       0        0        0      0       0      0     0      0      0
141782       0        0        0      0       0      0     0      0      0
215972       0        0        0      0       0      0     0      0      0
31759        0        0        0      0       0      0     0      0      0
30678        0        0        1      0       0      0     0      0      0
138353       0        0        0      0       0      0     0      0      0
406058       0        0        0      0       1      0     0      0      0
30405        0        0        0      0       0      1     1      0      0
1126         0        0        0      0       0      0     0      0      9
46043        0        0        0      0       0      0     0      3    431
512519       0        0        0      0       0      0     0      9      2
554594       0        0        0      0       0      0     0      0      6
210741       0        0        0      0       0      8     0      0      0
550229       0        0        0      0       0      0     0      0      3
105798       0        0        0      0       0      0     0      0      0
183473       0        0        0      0       0      0     0      0      0
541313       0        0        0      0       0      0     0      1      0
540305       0        2        0      1       1      1     2      4      5
511243       0        0        0      0       0      0     0      0      0
568829       0        0        0      0       0      0     0      0      0
185612       0        0        0      0       0      0     0      0      0
321018       0        0        0      0       0      0     0      4      1
549041       0        0        0      0       0      0     0      0      0
153762       0        0        0      0       0      0     0      0      1
155789       0        0        0      0       0      0     0      1      0
155495       0        0        0      0       0      0     0      1      0
1029         0        0        0      0       0      0     0      0      0
155526       0        0        0      0       0      0     0      0      1
197473       0        0        0      0       0      1     0      1      3
315545       1        0        0      2       2      0     1    117    727
348549       0        0        0      0       0      0     1     47    771
559394       0        0        0      0       0      0     0      1      2
341551       0        0        0      0       0      0     0      0      0
108964       0        1        0      1       0      1     3      7      1
330416       0        0        0      0       0      0     0      4      0
573586       0        0        0      0       0      0     0      0      0
568724       0        0        0      0       0      0     1     10      2
175045       0        3        0      0       1      2     1      6      3
552540       0        0        4      0       1      3     0      0      0
546313       0        0        0      1       0      0     0      1      0
548602       0        0        0      0       0      0     0      0      0
564501       0        0        0      0       0      1     0      0      0
47778        0        0        0      0       0      0     0      0      0
54107        0        0        0      0       0      0     0      0      0
25116        0        0        0      0       0      0     0      0      0
71074        0        1        1      0       1      0     0      1      0
252853       0        0        0      0       0      0     0      0      0
100679       0        1        0      0       0      1     0      0      0
540349       0        0        0      0       0      0     0      0      0
1140         0        0        0      0       0      0     0      0      0
101071       0        1        0      0       0      0     0      0      0
1143         0        0        0      0       0      0     0      0      0
141739       0        0        0      0       0      0     0      0      0
1141         0        0        0      0       0      0     0      0      0
237125       0        0        0      0       0      0     0      0      0
1144         0        0        0      0       0      0     0      0      0
512943       0        0        0      0       0      0     0      0      0
235345       0        0        0      0       1      2     0      0      0
141417       1        0        0      0       1      0     0      0      0
268471       0        0        0      0       0      2     0      0      0
395774       0        0        0      0       1      3     0      0      0
142115       0        0        0      3      81     72     1      0      0
239672       0        0        0      0       0      0     0      0      0
462844       0        0        0      0       0      0     0      0      0
243675       0        0        0      0       0      0     0      0      0
444679       0        0        0      0       0      0     0      0      0
540290       0        0        0      0       0      0     0      0      0
523571       0        0        0      2      26     36     0      0      0
545095       0        0        0      1       1      4     0      0      0
105679       0        0        0      0      18     11     0      0      0
105066       0        0        0      0       1      1     0      0      0
515962       0        0        0      1       6     10     0      0      0
525569       0        1        0      1      14     18     1      0      0
238793       0        0        0     17     196    246     0      0      0
538819       0        0        0      2      26     30     0      0      0
515691       0        0        2     18     187    213     1      0      0
525464       0        0        0     10      94    114     1      0      0
529521       0        0        0      1      12     23     0      0      0
202343       0        0        0      0       0      0     0      0      0
455926       0        0        1      8     122    139     0      0      0
246371       1       10       13    143    1730   1874    10      0      0
282132       0        0        0      0      27     30     0      0      0
254458       2        4        2     63     796    840     4      0      0
245697       1       19       31    646    6350   6459    42      2      3
45742        0        1        0     28     321    205     2      0      0
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163227       0        0        0      0       1      1     0      3      1
81643        0        0        0      4       6      9     2     66      8
531807       0        0        0      0       0      0     2     96      2
146764       1        0        0      5       0      0     1    539     13
158539       0        0        0      1       0      0     0     36      4
248182       0        1        0      2       1      0     4    113     25
235556       0        0        0      0       0      0     0      4      1
392867       0        0        0      3       0      0     2    193     34
8849         0        0        0      0       0      0     0     12      6
8854         0        0        0      0       0      0     0     40     15
272511       1        0        0      0       2      3     4     12     20
334678       0        0        0      1       2      0    10    212      6
46271        0        0        0      1       0      1     5    115      6
319651       0        0        0      0       0      0     0      1      0
255639       0        0        0      0       0      0     5    264      9
68895        0        0        0      0       0      0     3     14      5
108410       0        0        0      0       0      0     0     10      0
230360       0        0        0      0       0      0     1      7      0
71462        0        0        0      0       0      0     0      3      0
82812        0        0        0      0       0      0     0      1      0
325564       0        0        0      0       0      0     0      0      1
230735       0        0        0      0       0      0     0      1      4
224717       0        0        0      0       0      0     0      0      1
21967        0        0        0      0       0      0     0      0      1
362563       2        0        0      1       2      0    30    185     99
346453       0        0        0      0       0      0     0      2      0
586740       0        0        0      0       1      0    13     73     51
260031       0        0        0      0       0      0     0      0      0
236190       0        0        0      0       0      0     0      2      0
356544       0        0        0      0       0      0     0      7      1
228086       0        0        0      0       0      0     0      1      0
70306        0        0        0      1       0      0     4    280     12
128050       0        0        0      0       0      0     0     12      2
245610       0        0        0      0       0      0     0      7      0
141151       0        0        0      0       0      0     0      2      0
554703       0        0        0      1       0      0     0     33      4
169940       0        0        0      1       1      0    18    290    105
93965        0        0        0      1       0      0     0      7      1
277789       0        0        0      0       0      0     0      4      0
106027       0        0        0      0       0      0     0      0      1
91588        0        0        0      0       0      0     0      0      1
523223       0        0        0      0       0      0     0      2      1
536526       0        0        0      0       0      0     0      2      0
84937        0        0        0      2       0      1    25    128     51
9286         0        0        0      0       0      1     4     31     21
60374        0        0        0      0       0      0     1     10      9
327981       0        0        0      0       0      0     0      1      0
234088       0        0        0      0       0      0     0      1      0
353304       0        0        0      0       0      0     0      1      0
536960       0        0        0      0       0      0     0      1      0
9176         0        0        0      0       0      0    21     12     70
91087        0        0        0      0       0      0     0      0      1
198609       0        0        0      0       0      0     0      1      0
9179         0        0        0      0       0      0     0      1      1
231428       0        0        0      0       0      0     3     20      9
559028       0        0        0      0       1      0    15     16      1
232634       0        0        0      0       0      0     0      2      0
230146       0        0        0      0       0      0     0      1      0
30925        0        0        0      3       0      0     3    530      5
111212       0        0        0      1       0      0     0      6      0
549366       0        2        3     15       3      0    75   1787    194
233630       0        0        0      0       0      0     0      4      1
220372       0        0        0      0       0      0     0      1      0
250349       0        0        0      0       0      0     0      2      1
339560       0        0        0      0       0      0    14     13      1
579117       0        0        0      4       0      0     1    190      6
9250         0        0        0      0       0      0     0      6      0
559495       0        0        0      0       0      0     0      6      2
320634       0        0        0      0       0      0     0      2      0
306938       0        0        0      0       0      0     1      9      0
534155       0        0        0      0       0      0     0      0      0
196433       0        0        0      0       0      0     0      2      0
553472       0        0        0      1       0      0     2     21      2
573179       0        0        0      0       0      0     2     42      0
227760       0        0        0      0       0      0     1     19      0
160210       0        0        0      0       0      0     0      2      0
579828       0        0        0      0       0      0     0      0      0
171395       0        0        0      0       0      0     0      2      0
160207       0        0        1      1       1      1    25    163     30
159955       0        0        0      1       0      1     8      8      4
245715       0        0        0      1       0      1     0      1      0
351250       0        0        0      0       0      0     0      2      5
567653       1        1        1      1       1      0    12    167     25
114914       0        0        0      0       0      0     0      0      0
160550       0        0        0      1       2      0     4    108      2
20588        0        0        0      1       1      0     6     12      2
132680       0        0        0      1       0      0     0     17      4
570650       0        0        0      0       0      0     0      3      0
9142         0        0        0      1       0      0     0     20      6
160832       0        0        0      0       0      0     0      1      0
549933       0        0        0      0       0      0     2     10      1
111916       0        0        0      1       0      0     3     73      8
45389        0        0        0      0       0      0     3     86      2
9129         0        0        0      0       0      0     0      0      0
9128         0        0        0      0       0      0     6     28      6
149694       0        0        0      0       1      0    17     25     35
236039       0        0        0      0       0      0     0     53      6
246710       0        1        0      0       0      0     0      1      1
9036         0        0        0      0       0      0     0      9      0
349841       0        0        0      0       0      0     0      0      1
267927       0        0        0      1       0      0     0     24      4
229619       0        0        0      0       0      0     1      0      0
83672        0        0        0      1       1      0     2     53      3
346602       0        0        0      1       0      0     0     15      0
143206       0        0        0      0       0      0     0      3      1
148004       0        0        0      0       0      0     0      1      0
151850       0        0        0      1       0      0     0     88      1
179131       0        1        0      0       0      0     0      0      0
132764       0        0        0      0       0      1     0      0      0
133572       0        0        0      0       0      0     0      1      0
38945        0        0        0      0       0      0     0      0      0
113783       0        0        0      1       0      0     0      0      0
276135       0        0        0      0       0      0     0      3      0
141639       0        0        1      0       0      0     0      5      1
173543       0        0        0      0       0      0     0      0      0
238071       0        0        3      0       0      0     0      1      1
586719       0        1        0      4      14      9     1      1      0
139040       0        0        0      2       3      1     0      0      1
140647       0        0        0      0       0      0     1      0      0
256999       0        0        0      1       2      2     2      0      2
155961       0        0        0      0       0      0     0      1      0
168803       0        0        0      0       0      0     1      1      1
341133       0        0        0      0       0      0     0      1      0
128057       0        0        0      1       1      0     0    135      2
9123         0        0        0      0       0      0     1      0      0
237124       0        0        0      1       0      2     0      0      0
571322       0        0        0      1       0      0     0      0      0
252198       0        0        0      0       0      0     0      5      1
543247       0        0        0      0       0      0     0      0      0
104600       0        0        0      0       0      0     0      6      1
576068       0        0        0      0       0      0     0     62      1
357877       0        3        1      1       0      0     0      0      0
168069       1      218       32     21       6      1     0      2      1
161901       0        1        0      0       0      0     0      0      0
215466       0        0        0      0       0      0     0      0      0
423025       0        0        0      0       0      0     0      0      0
509473       0        0        0      0       0      0     0      0      0
9458         0        0        0      0       0      0     0      0      0
583215       0        0        0      0       0      0     0      0      0
578335       0        0        0      0       0      0     0      0      1
9459         0        0        0      0       0      0     0      1      0
70512       53        3        0      3       0      1     0      1      3
9514      2371      476       33     79      42     43     9     27    222
29369        0        0        0      0       0      0     0      0      0
471414       0        0        0      0       0      0     0      0      0
9466         0        0        0      1       0      0     0      0      0
286210       0        0        0      0       0      1     0      0      0
       TRRsed1 TRRsed2 TRRsed3  TS28   TS29  Even1  Even2  Even3
549322       0       0       0     0      0      0      0      0
522457       0       0       0     0      0      0      0      0
951          0       0       0     0      0      0      0      0
244423       0       0       0     0      0      0      0      0
586076       0       0       0     0      0      0      0      0
246140       0       0       0     0      0      0      0      0
143239       0       0       0     0      0      0      0      0
244960       0       0       0     0      0      0      0      0
255340       0       0       0     0      0      2      1      0
144887       0       0       0     0      0      0      0      0
141782       0       0       0     0      0      0      0      0
215972       0       0       0     0      0      0      0      0
31759        0       0       0     0      0      0      0      0
30678        0       0       0     0      0      0      0      0
138353       0       2       0     0      0      0      0      0
406058       0       0       0     0      0      0      0      0
30405        0       0       0     0      0      6      0      0
1126         0       0       0     0      0      0      0      0
46043        0       1       2     0      0      0      0      0
512519       0       0       0     0      0      0      0      0
554594       0       0       1     0      0      0      0      0
210741       0       0       0     0      0      0      0      0
550229       0       0       0     0      0      0      0      0
105798       0       3       2     0      0      0      0      0
183473       0       2       5     0      0      0      0      0
541313       0       0       1     0      0      0      0      0
540305      63    1320    2305     4      0     22      0      0
511243       0       2       7     0      0      0      0      0
568829       0       0       1     0      0      0      0      0
185612       1      21      25     0      0      0      0      0
321018       0       9     116     0      0      0      0      0
549041       0       1       3     0      0      0      0      0
153762       0       0       0     0      0      0      0      0
155789       0       0       0     0      0      0      0      0
155495       0       0       0     0      0      0      0      0
1029         0       0       1     0      0      0      0      0
155526       3      37     426     1      0      5      1      0
197473       0       2      98     0      0      0      0      0
315545     217     921     694     0      0      1      0      1
348549     176     440     340     0      0      4      1      0
559394      19       8       5     0      0      0      0      0
341551       0       2       1     0      0      0      0      0
108964     166    3437    6404    10      3     21      0      2
330416       0       0       0     0      0      0      0      0
573586       0       1       2     0      0      0      0      0
568724       3     151     117     0      0      1      0      0
175045      83    1692    3030     5      0     27      1      0
552540       0       0       0     0      0      0      0      0
546313       9      20      11     0      1      2      2     29
548602       0       3       1     0      0      0      0      0
564501       0       0       0     0      0      0      0      0
47778        0       4       3     0      0      0      0      2
54107        2      25      17     1      1      0      0      4
25116        0      11       2     0      0      1      0      0
71074        9     118      51     0      1      4      3     30
252853       0       1       2     0      0      0      0      0
100679       0      33      20     2      0      0      0      2
540349       0       4       3     0      0      0      0      2
1140         0       3       1     0      0      0      0      1
101071       0       4       1     0      0      0      0      0
1143         0       0       1     0      0      0      0      0
141739       0       1       0     0      0      0      0      0
1141         0       1       0     0      0      0      0      0
237125       0      12      10     0      0      0      0      0
1144         1      17       3     0      0      0      2      1
512943       0      14      11     0      0      0      0      1
235345       0       1       0     0      0      0      0      0
141417       1      14       2     0      0      0      0      2
268471       0       0       0     0      0      0      0      0
395774       0       0       0     0      0      0      0      0
142115       0       0       0     0      0      0      0      0
239672       5      14      66     0      0      0      0      0
462844       3      21      30     0      0      0      0      0
243675       0       0      23     0      0      0      0      0
444679       0       0      19     0      0      0      0      0
540290       2       5      14     0      0      0      0      0
523571       0       0       0     0      0      0      0      0
545095       0       0       0     0      0      0      0      0
105679       0       0       0     0      0      0      0      0
105066       0       0       0     0      0      0      0      0
515962       0       0       0     0      0      0      0      0
525569       0       0       0     0      0      0      0      0
238793       1       0       0     0      0      0      0      0
538819       0       0       0     0      0      0      0      0
515691       0       0       0     0      0      0      0      0
525464       0       0       0     0      0      0      0      0
529521       0       0       0     0      0      0      0      0
202343       0       3       0     0      0      0      0      0
455926       0       0       2     0      0      0      0      0
246371       1       1       1     0      1      2      0      0
282132       0       0       0     0      0      0      0      0
254458       1       0       0     0      0      1      0      0
245697       0       2       0     0      0      6      1      1
45742        0       1       0     0      0      0      0      0
441638       0       0       1     0      0      0      0      0
251993       0       0       0     0      0      0      0      0
462098       0       0       0     0      0      1      0      0
570593       0       0       0     0      0      0      0      0
159421       0       0       1     0      0      0      0      0
104332       0       2      86     0      1      0      0      0
137608       0       0       0     0      0      0      0      0
200619       0       1     233     1      0      2      0      0
568129       0       0       9     0      0      0      0      0
348720       1       0       3     0      0      1      0      0
528115       0       0       0     0      0      0      0      0
257655       0       2       2     0      0      0      0      0
173903       0       0       1     0      0      0      0      0
47016        0       0       1     0      0      0      0      0
548723       0       0       1     0      0      0      0      0
542636       0       0       1     0      0      0      0      0
526502       0       0       1     0      0      0      0      0
311512       0       0       1     0      0      0      0      0
132088       0       0       0     0      0      0      0      0
202347       0       0       0     0      0      0      0      0
152431       0       0       0     0      0      0      0      0
272691       0       0       0     0      0      0      0      0
236606       0       0       0     0      0      0      0      0
525          0       0       0     0      0      0      0      0
52710        0       0       0     0      0      0      0      0
592689       0       1       0     0      0      0      0      0
55681        0       0       0     0      0      0      0      0
206978       0       0       0     0      0      0      0      0
467541       0       0       1     0      0      0      0      0
566371       0       0       1     0      0      0      0      0
453468       0       0       0     0      0      0      0      0
557121       0       0       0     0      0      0      0      0
524986       0       0       0     0      0      0      0      0
102663       0       0       0     0      0      0      0      0
264985       0       0       0     0      0      0      0      0
37119        0       0       0     0      0      0      0      0
349670       0       0       0     0      0      0      0      0
240012       0       0       0     0      0      0      0      0
248224       0       0       0     0      0      0      0      0
236057       0       0       0     0      0      0      0      0
236619       0       0       0     0      0      0      0      0
565033       0       0       0     0      0      0      0      0
212360       0       0       0     0      0      0      0      0
146124       1       0       0     0      0      0      0      0
251552       1       3       4     0      0      0      0      0
542257       0       0       0     0      0      0      0      0
548984       0       0       0     0      0      0      0      0
201596       0       0       0     0      0      0      0      0
158026       0       0       2     0      0      0      0      0
62881        0       0       0     0      0      3      0      0
329648       0       0       0     0      0      0      0      0
570725       0       0       0     0      0      0      0      0
206080       0       0       0     0      0      0      0      0
547371       0       0       0     0      0      0      1      0
140729       0       0       0     0      0      1      0      0
347836       0       0       0     0      0      0      0      0
76707        0       0       0     0      0      0      0      0
136107       0       0       0     0      0      0      0      0
170941       0       0       0     0      0      0      0      0
155703       0       0       0     0      0      0      0      0
213630       0       0       0     0      0      0      0      0
546805       0       0       0     0      0      0      0      0
448625       0       1       2     0      0      0      0      0
101828       0       0       0     0      0      0      0      0
319002       0       2       0     2      1     21      5      1
219191       0       0       0     0      0      0      0      0
410763       0       0       0     0      0      0      0      0
412056       0       0       0     0      0      1      0      0
550661       0       0       0     0      0      0      0      0
550655       0       0       0     0      0      0      0      0
526          0       0       0     0      0      0      0      0
515698       0       0       2     0      0      0      0      0
379145       0       0       0     0      0      0      0      0
245798       0       0       0     0      0      0      0      0
583469       0       0       0     0      0      0      0      0
254858       0       0       0     0      0      0      0      0
407348       0       0       1     0      0      0      0      0
250392      56      14       1     3      1     10      5      8
148407       0       0       0     0      0      0      0      0
104364       0       0       0     0      0      0      0      0
250570       0       0       0     0      0      0      0      0
308755       1       0       0     0      0      0      0      0
147601       0       0       0     0      0      0      0      0
255494       8       2       0     0      0      0      0      0
146866       2       1       0     0      1      0      0      0
155847       4       0       0     0      1      0      0      1
715          2       0       0     0      0      0      0      0
32110        0       0       0     0      0      0      0      0
140297       0       0       0     0      0      0      0      0
105600       0       0       0     0      0      0      0      0
40123        0       0       0     0      0      0      0      0
79           0       0       0     0      0      0      0      0
18599        0       0       0     0      0      0      0      0
77878        1       1       0     1      2      2      0      1
101553       0       0       1     0      1      0      0      0
204          0       0       0     0      0      0      0      0
134          0       0       0     0      0      1      0      0
551498       0       0       0     0      0      0      0      0
99797        0       0       0     0      0      0      0      0
150294       0       0       0     0      0      0      0      0
155634       0       1       0     0      0      0      0      0
153917       0       0       1     0      0      0      0      0
348180       0       0       0     1      0      0      0      0
138          0       0       0     0      0      0      0      0
547463       0       1       2     0      0      0      0      0
152271       0       0       0     0      0      0      0      0
227785       0       1      11  1566   1668      2      0      2
104804       0       0       0     1      0      0      0      0
128          0       0       0     0      2      0      0      0
228569       0       0       0     0      0      0      0      0
100449       0       0       0     0      3      0      0      0
262662       0       0       0     0      2      0      0      0
246686       0       0       0     0      0      0      0      0
166443       0       0       0     0      0      0      0      0
368907       0       0       0     0      0      0      0      0
12724        0       0       0     0      0      0      0      0
93749        0       0       0     0      0      0      0      0
378053       0       0       0     0      0      0      0      0
165106       0       0       0     0      0      0      0      0
135424       0       0       0     0      0      0      0      0
167566       0       0       0     0      0      0      0      0
12751        0       0       0     0      0      0      0      0
12753        0       0       0     0      0      0      0      0
560734       0       0       0     0      0      0      0      0
231733       0       0       0     0      0      0      0      0
145101       0       0       0     0      0      0      0      0
367851       0       0       0     0      0      0      0      0
275357       0       0       0     0      0      0      0      0
315847       0       0       0     0      0      0      0      0
428663       0       0       0     0      0      0      0      0
165629       0       0       0     0      0      0      0      0
243161       0       0       0     0      0      0      0      0
525323       0       0       0     0      0      0      0      0
157893       0       0       0     0      0      0      1      0
172332       0       0       0     0      0      0      0      0
179479       0       0       0     0      0      0      0      0
145776       0       2       1     0      1      0      0      0
263308       0       6       4     0      0      0      0      0
341901       0       1       0     0      0      0      0      0
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113783       0       0       0     0      0      0      0      0
276135       0       0       0     0      0      0      0      0
141639       0       0       0     0      0      0      0      0
173543       1       0       0     0      0      0      0      0
238071       0       0       0     0      0      0      0      0
586719       0       7       4     0      0      1      0      0
139040       0       3       0     0      0      0      0      0
140647       0       4       0     0      0      0      0      0
256999       0       0       0     0      0      0      0      0
155961       0       0       0     0      0      0      0      0
168803       0       0       0     0      0      0      0      0
341133       0       0       0     0      0      0      0      0
128057       0       0       0     0      0      0      0      0
9123         0       0       0     0      0      0      0      0
237124       0       1       0     0      0     18     14     12
571322       0       0       0     0      0      0      0      0
252198       0       1       0     0      0      0      0      0
543247       0       1       0     0      0      2      1      1
104600       0       0       0     0      0      0      0      0
576068       0       0       0     0      0      0      1      0
357877       1       0       1     0      1     29     20     28
168069       1       1       2     2      0    107     67     60
161901       0       0       0     0      0      0      1      0
215466       0       0       0     0      0      0      0      1
423025       0       0       0     0      0      1      0      0
509473       0       0       0     0      0      0      0      0
9458         0       0       0     0      0      0      0      2
583215       0       0       0     0      0      0      0      0
578335       0       0       0     0      0      0      0      0
9459         0       0       0     0      0      0      0      0
70512        1       1       0     0      0      0      3      2
9514         9      16      10    22      8     28     37     20
29369        0       0       0     0      0      0      0      0
471414       0       0       0     0      0      0      0      0
9466         0       0       0     0      0      0      0      0
286210       0       0       0     0      0      0      0      0
 [ reached 'max' / getOption("max.print") -- omitted 15370 rows ]

colData(tse)
DataFrame with 26 rows and 7 columns
        X.SampleID   Primer Final_Barcode Barcode_truncated_plus_T
          <factor> <factor>      <factor>                 <factor>
CL3        CL3      ILBC_01        AACGCA                   TGCGTT
CC1        CC1      ILBC_02        AACTCG                   CGAGTT
SV1        SV1      ILBC_03        AACTGT                   ACAGTT
M31Fcsw    M31Fcsw  ILBC_04        AAGAGA                   TCTCTT
M11Fcsw    M11Fcsw  ILBC_05        AAGCTG                   CAGCTT
...            ...      ...           ...                      ...
TS28         TS28   ILBC_25        ACCAGA                   TCTGGT
TS29         TS29   ILBC_26        ACCAGC                   GCTGGT
Even1        Even1  ILBC_27        ACCGCA                   TGCGGT
Even2        Even2  ILBC_28        ACCTCG                   CGAGGT
Even3        Even3  ILBC_29        ACCTGT                   ACAGGT
        Barcode_full_length SampleType
                   <factor>   <factor>
CL3             CTAGCGTGCGT      Soil 
CC1             CATCGACGAGT      Soil 
SV1             GTACGCACAGT      Soil 
M31Fcsw         TCGACATCTCT      Feces
M11Fcsw         CGACTGCAGCT      Feces
...                     ...        ...
TS28            GCATCGTCTGG      Feces
TS29            CTAGTCGCTGG      Feces
Even1           TGACTCTGCGG      Mock 
Even2           TCTGATCGAGG      Mock 
Even3           AGAGAGACAGG      Mock 
                                       Description
                                          <factor>
CL3     Calhoun South Carolina Pine soil, pH 4.9  
CC1     Cedar Creek Minnesota, grassland, pH 6.1  
SV1     Sevilleta new Mexico, desert scrub, pH 8.3
M31Fcsw M3, Day 1, fecal swab, whole body study   
M11Fcsw M1, Day 1, fecal swab, whole body study   
...                                            ...
TS28                                       Twin #1
TS29                                       Twin #2
Even1                                      Even1  
Even2                                      Even2  
Even3                                      Even3  

tse <- tse[1:5, c(1, 4)]
colData(tse)
DataFrame with 2 rows and 7 columns
        X.SampleID   Primer Final_Barcode Barcode_truncated_plus_T
          <factor> <factor>      <factor>                 <factor>
CL3        CL3      ILBC_01        AACGCA                   TGCGTT
M31Fcsw    M31Fcsw  ILBC_04        AAGAGA                   TCTCTT
        Barcode_full_length SampleType                              Description
                   <factor>   <factor>                                 <factor>
CL3             CTAGCGTGCGT      Soil  Calhoun South Carolina Pine soil, pH 4.9
M31Fcsw         TCGACATCTCT      Feces M3, Day 1, fecal swab, whole body study

Exercises

From OMA online book, Chapter 3: Data containers

  • All exercises

References

https://microbiome.github.io/

Huang, Ruizhu, Charlotte Soneson, Felix G. M. Ernst, et al. 2021. “TreeSummarizedExperiment: A S4 Class for Data with Hierarchical Structure.” F1000Research 9: 1246. https://doi.org/10.12688/f1000research.26669.2.
Huber, W., V. J. Carey, R. Gentleman, S. Anders, M. Carlson, B. S. Carvalho, H. C. Bravo, et al. 2015. “Orchestrating High-Throughput Genomic Analysis with Bioconductor.” Nature Methods 12 (2): 115–21. http://www.nature.com/nmeth/journal/v12/n2/full/nmeth.3252.html.
Ramos, Marcel, Lucas Schiffer, Angela Re, Rimsha Azhar, Azfar Basunia, Carmen Rodriguez, Tiffany Chan, et al. 2017. “Software for the Integration of Multiomics Experiments in Bioconductor.” Cancer Research 77 (21): e39–42. https://doi.org/10.1158/0008-5472.CAN-17-0344.